Journal: Journal of Orthopaedic Translation
Article Title: Extracellular vesicles originating from induced pluripotent stem cell-derived chondrocytes facilitate the regeneration of osteoarthritic cartilage
doi: 10.1016/j.jot.2025.101035
Figure Lengend Snippet: iChondrocyte-sEVs-derived SRR facilitated cartilage regeneration via the P38/ERK pathway. a Representative histopathological image of SRR in articular cartilage (n = 6). Scale bar: 200 μm b Western Blot analysis of SRR levels in iPSC-sEVs, iMSC-sEVs and iChondrocyte-sEVs (n = 3). c Western Blot analysis of SRR, SOX9, COLII expression in MSC-derived chondrocytes treated with or without iChondrocyte-sEVs (n = 3). d Western Blot analysis of key proteins in the P38/ERK signaling pathway in MSC-derived chondrocytes treated with or without iChondrocyte-sEVs (n = 3). e Schematic diagram illustrating the molecular mechanism by which iChondrocyte-sEVs exert their regenerative effects. All groups except the sham group were conducted under DMM conditions.
Article Snippet: The following primary antibodies were used for western blot analysis: CD9 (RGAB101, Rengen Biosciences, China), CD63 (RGAB103, Rengen Biosciences, China), Alix (RGAB100, Rengen Biosciences, China), SRR (17955-1-AP, Proteintech, China), SOX9 (GB11280, Servicebio, China), COLII (28459, Proteintech, China), ACAN (68350, Proteintech, China), β-actin (GB15003, Servicebio, China), SynGAP (19739, Proteintech, China), JNK1 ( A23206 , Abclonal, China), P-JNK (AP1337, Abclonal, China), P38 ( GB154685 , Servicebio, China), P-P38 ( GB153380 , Servicebio, China), ERK (4695, CST, USA), and P-ERK(4370, CST, USA).
Techniques: Derivative Assay, Western Blot, Expressing
